Journal: ADMET & DMPK

Article Title: Anti-inflammatory potential of plant-derived extracellular vesicles from Solanum nigrum L. integrated in gelatine-dopamine hydrogel on RAW 264.7 and MC3T3 cells

doi: 10.5599/admet.3149

Figure Lengend Snippet: Interleukin-6 (IL-6) concentration by (A) RAW 264.7 and (B) MC3T3 cells LPS induction and PDEV (2.5, 5 and 10 μg mL -1 ) treatment released from Gel-Dop hydrogel. Data presented as mean ± standard deviation (*** p < 0.001, **** p <0,0001)

Article Snippet: Other materials used in this study were Dulbecco’s modified eagle medium (DMEM) - high glucose, foetal bovine serum (FBS), Antibiotic-Antimycotic (ABAM), polyethylene glycol (PEG) 6000, trehalose (Sigma-Aldrich; Merck), phosphate-buffered saline (PBS), sodium periodate (NaIO 4 ) (Sigma-Aldrich), bovine serum albumin (BSA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), dimethyl sulfoxide (DMSO), paraformaldehyde (PFA), kit BCA Assay (PierceTM BCA Protein Assay Kit, Thermo Scientific), PKH67 Green Fluorescent Cell Linker Kits (Sigma-Aldrich; Merck), and Mouse Interleukin-6 (IL-6) ELISA Kit (BT Lab, Cat. No. E0049Mo).

Techniques: Concentration Assay, Standard Deviation

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 2 Microscopy picture of MDCK cells (10 objective). Non-infected cells and infected cells showing cytopathic effect with 50–80% cells detached or >80% cells detached are shown (as indicated)

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Microscopy, Infection

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 4 (a) Exemplified design of mutagenesis targeting a region in the PB1 segment of a H5N1 virus. Template is in gray and forward and reverse primers are in orange and blue, respectively. Mutagenesis site is indicated in red. (b) Western blot confirmation of viral gene deletion. Cell culture lysates of MDCK cells infected for 24 h with MOI 5 wild-type (wt) or PB1-F2-deficient (ΔF2) virus were run on a 12% SDS-PAGE, blotted on PVDF membranes, and probed against influenza A virus nucleoprotein (NP), PB1-F2, and actin

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Mutagenesis, Virus, Western Blot, Cell Culture, Infection, SDS Page

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 5 (a) MDCK cells in the density required for plaque assays. (b) Plaque phenotypes: plaque assays 48 h postinfection, after fixation, and crystal violet staining. Virus is diluted tenfold between each column, from left to right. (c) Plaque purification. Single plaques are marked from the bottom of the well. Virus from individual plaques is aspirated with a sterile pipet

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Staining, Virus, Sterility

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 4 Summary of an influenza virion proteome. Viral and host proteins identified and quantified in a sample of MDCK-grown influenza A/Puerto Rico/8/1934 virus, using the methods described in this chapter

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Virus

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 2 Examples of smFISH analysis. (a) Images of MDCK cells infected with influenza virus (PR8 strain) at MOI ¼ 5 and fixed at 6 h postinfection. Two probe sets (24 Cy3-labeled probes, 24 Cy5-labeled probes) targeting different regions of the NA vRNA were used for hybridization. Maximum intensity projections of 3D fluorescent stack images are shown for each color channel. The bottom right panel is the merged fluorescent image of DAPI, Cy3, and Cy5 channels (scale bar ¼ 10 μm). (b) Magnified images of the squared regions in (a) are shown on the top. The corresponding detected spots for each channel image are shown on the bottom. This experiment serves as a positive control for smFISH colocalization analysis. (c) Images of MDCK cells infected with influenza virus (PR8 strain) at MOI ¼ 5 and fixed at 8 h postinfection. The infected cells were hybridized using Cy5-labeled probes against the NA vRNA and Cy3-labeled probes against host β-actin mRNA. Maximum intensity projections of the 3D fluorescent stack images are shown for each color channel. The merged fluorescent image of DAPI, Cy3, and Cy5 channels is shown on the bottom right (scale bar ¼ 10 μm). (d) Magnified images of the squared regions in (c) are shown on the top. The corresponding detected spots for each channel image are shown on the bottom. This experiment serves as a negative control for RNA FISH colocalization analysis

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Infection, Virus, Labeling, Hybridization, Positive Control, Negative Control

Journal: Methods in Molecular Biology

Article Title: Influenza Virus

doi: 10.1007/978-1-4939-8678-1

Figure Lengend Snippet: Fig. 1 Scheme of a fluorescent vRNP by fusing eGFP to the PA polymerase component. (a) Schematic of vRNP with PA-eGFP protein and (b) visualization of an MDCK cell infected with WSN PA-eGFP (MOI ¼ 3) at 8 hpi. The localization is diffuse in the nucleus with punctate cytoplasmic foci; PA-eGFP (tagged viral protein) is shown in green and DAPI stain (nucleus) in blue. Scale bar: 5 μm

Article Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC, PTA-6500).

Techniques: Infection, Staining